Molecular Biology of the Gene

出版者:Benjamin Cummings

作者:James D. Watson

页数:830

出版时间:2004-01-09

价格:CAD 143.95

装帧:Hardcover

isbn号码:9780805346350

具体描述

Every student package automatically includes a CD-ROM and access to a website containing tutorials, molecular models, and more.

The long-awaited Fifth Edition of James D. Watson's classic text, Molecular Biology of the Gene, has been thoroughly revised and is published to coincide with the 50th anniversary of Watson and Crick's paper on the structure of the DNA double- helix.

Despite being completely updated, the new edition retains the distinctive character of earlier editions that made it the most widely used book in molecular biology. Twenty-one concise chapters, co-authored by five highly respected molecular biologists, provide current, authoritative coverage of a fast-changing discipline. The completely new art is printed in full colour for the first time.

Divided into four parts, the first (Genetics and Chemistry) begins with an overview of molecular biology, placing the discipline in historical context and introducing the basic chemical concepts that underpin our description of molecular biology today. The second part (Central Dogma) forms the heart of the book, describing in detail the basic mechanisms of molecular biology. The third part of the book (Regulation) deals with how gene expression is regulated - from the examination of basic mechanisms that regulate gene expression in bacterial and eukaryotic systems, to a description of how regulation of gene expression lies at the heart of the process of development. Recent findings from sequencing whole genomes of several animals have revealed that they all share essentially the same genes. The last chapter in the regulation section looks at how changes in gene regulation can account for how different animals can be made from the same genes. The final part of the book (Methods) deals with the techniques and methods used in molecular biology.

[Contents]

I. CHEMISTRY AND GENETICS.

1. The Mendelian View of the World.

2. Nucleic Acids Convey Genetic Information.

3. The Importance of Weak Chemical Interactions.

4. The Importance of High Energy Bonds.

5. Weak and Strong Bonds Determine Macromolecular Interactions.

II. CENTRAL DOGMA.

6. The Structures of DNA and RNA.

7. Chromatin and the Nucleosome.

8. The Replication of DNA.

9. The Mutability and Repair of DNA.

10. Homologous Recombination of DNA.

11. Site Specific Recombination and Transposition of DNA.

12. Mechanisms of Transcription.

13. The Processing of RNA.

14. Translation.

15. The Genetic Code.

III. REGULATION.

16. Gene Regulation in Prokaryotes.

17. Gene Regulation in Eukaryotes.

18. Gene Regulation in Development.

19. Comparative Genomics and the Evolution of Animal Diversity.

IV. METHODS.

20. Techniques of Molecular Biology.

21. Model Systems: Cells and Viruses.

[Features]

Early chapters on chemical and molecular interactions give a richer context to the material that follows and stress the importance of weak chemical interactions in biology.

The text has balanced coverage of prokaryotic and eukaryotic systems.

Important experiments and techniques relevant to chapter concepts are revealed in boxes in every chapter. These include recent techniques from the fields of genomics and bioinformatics.

Quantitative and critical thinking end-of-chapter questions allow students to apply their knowledge of the chapters key concepts.

An all new art programme features more than 750 line drawings in full colour. In addition, the art in the text has been integrated into animations on the accompanying CD-ROM and website.

The lively, incisive writing style that characterized earlier editions is once again a hallmark in the new edition.

Integrated media helps students better understand complex structures and molecular interactions. The CD-ROM and website offer animated tutorials, critical thinking exercises, and 3-D interactive molecular models in Chime.

作者简介

James D. Watson was Director of Cold Spring Harbor Laboratory from 1968 to 1993 and is now its President. He spent his undergraduate years at the University of Chicago and received his Ph.D. in 1950 from Indiana University. Between 1950 and 1953, he did postdoctoral research in Copenhagen and Cambridge, England. While at Cambridge, he began the collaboration that resulted in the elucidation of the double-helical structure of DNA in 1953. (For this discovery, Watson, Francis Crick, and Maurice Wilkins were awarded the Nobel Prize in 1962.) Later in1953, he went to the California Institute of Technology. He moved to Harvard in 1955, where he taught and did research on RNA synthesis and protein synthesis until 1976. He was the first Director of the National Center for Genome Research of the National Institutes of Health from 1989 to 1992. Dr. Watson was sole author of the first, second, and third editions of Molecular Biology of the Gene, and a co-author of the fourth edition. These were published in 1965, 1970, 1976, and 1987 respectively. Watson has also been involved in two other textbooks: he was one of the original authors of Molecular Biology of the Cell and is also an author of Recombinant DNA: a short course.

Tania A. Baker is the Whitehead Professor of Biology at the Massachusetts Institute of Technology and an Investigator of the Howard Hughes Medical Institute. She received a B.S. in biochemistry from the University of Wisconsin, Madison, and a Ph.D. in biochemistry from Stanford University in 1988. Her graduate research was carried out in the laboratory of Professor Arthur Kornberg and focused on mechanisms of initiation of DNA replication. She did postdoctoral research in the laboratory of Dr. Kiyoshi Mizuuchi at the National Institutes of Health, studying the mechanism and regulation of DNA transposition. Her current research explores mechanisms and regulation of genetic recombination, enzyme-catalyzed protein unfolding, and ATP-dependent protein degradation. Professor Baker received the 2001 Eli Lilly Research Award from the American Society of Microbiology and the 2000 MIT School of Science Teaching Prize for Undergraduate Education. She is co-author (with Arthur Kornberg) of the book DNA Replication, Second Edition.

Stephen P. Bell is a Professor of Biology at the Massachusetts Institute of Technology and an Assistant Investigator of the Howard Hughes Medical Institute. He received B.A. degrees from the Department of Biochemistry, Molecular Biology, and Cell Biology and the Integrated Sciences Program at Northwestern University and a Ph.D. in biochemistry at the University of California, Berkeley in 1991. His graduate research was carried out in the laboratory of Robert Tjian and focused on eukaryotic transcription. He did postdoctoral research in the laboratory of Dr. Bruce Stillman at Cold Spring Harbor Laboratory, working on the initiation of eukaryotic DNA replication. His current research focuses on the mechanisms controlling the duplication of eukaryotic chromosomes. Professor Bell received the 2001 ASBMB-Schering Plough Scientific Achievement Award, and the Everett Moore Baker Memorial Award for Excellence in Undergraduate Teaching at MIT in 1998.

Alexander Gann is Editorial Director of Cold Spring Harbor Laboratory Press, and a faculty member of the Watson School of Biological Sciences at Cold Spring Harbor Laboratory. He received his B.Sc in microbiology from University College London and a Ph.D. in molecular biology from The University of Edinburgh in 1989. His graduate research was carried out in the laboratory of Noreen Murray and focused on DNA recognition by restriction enzymes. He did postdoctoral research in the laboratory of Mark Ptashne at Harvard, working on transcriptional regulation, and that of Jeremy Brockes at the Ludwig Institute of Cancer Research at University College London, where he worked on newt limb regeneration. He was a Lecturer at Lancaster University, England, from 1996 to 1999, before moving to Cold Spring Harbor Laboratory. He is co-author (with Mark Ptashne) of the book Genes & Signals (2002).

Michael Levine is a Professor of Molecular and Cell Biology at the University of California, Berkeley, and is also Co-Director at the Center for Integrative Genomics. He received his B.A. from the Department of Genetics at U.C. Berkeley, and his Ph.D. with Alan Garen in the Department of Molecular Biophysics and Biochemistry from Yale Universityin 1981. As a postdoctoral fellow with Walter Gehring and Gerry Rubin from 1982- 1984, he studied the molecular genetics of Drosophila development. Professor Levine's research group currently studies the gene networks responsible for the gastrulation of the Drosophila these two species should be italicized and Ciona (sea squirt) embryos. He holds the F. Williams Chair in Genetics and Development at U.C. Berkeley. He was awarded the Monsanto Prize in Molecular Biology from the National Academy of Sciences in 1996, and was elected to the American Academy of Arts and Sciences in 1996 and the National Academy of Sciences in 1998.

Richard M. Losick is the Maria Moors Cabot Professor of Biology, a Harvard College Professor, and a Howard Hughes Medical Institute Professor in the Faculty of Arts & Sciences at Harvard University. He received his A.B. in chemistry at Princeton University and his Ph.D. in biochemistry at the Massachusetts Institute of Technology. Upon completion of his graduate work, Professor Losick was named a Junior Fellow of the Harvard Society of Fellows when he began his studies on RNA polymerase and the regulation of gene transcription in bacteria. Professor Losick is a past Chairman of the Departments of Cellular and Developmental Biology and Molecular and Cellular Biology at Harvard University. He received the Camille and Henry Dreyfuss Teacher-Scholar Award, is a member of the National Academy of Sciences, a Fellow of the American Academy of Arts and Sciences, a Fellow of the American Association for the Advancement of Science, a Fellow of the American Academy of Microbiology, and a former Visiting Scholar of the Phi Beta Kappa Society.

读后感

作为基础知识的梳理和学习之用，这本书无疑是很给力的。 从章节架构内含的相互联系可以看出作者和编辑很是努力了一把的。 任何具备一些常识的人想要进入分子生物学专业都可以从这本书的序章开始，一点一点，一步一步深入进去。 当合上书本，那些巧妙地机制原理生动地在脑中一一...

作为基础知识的梳理和学习之用，这本书无疑是很给力的。 从章节架构内含的相互联系可以看出作者和编辑很是努力了一把的。 任何具备一些常识的人想要进入分子生物学专业都可以从这本书的序章开始，一点一点，一步一步深入进去。 当合上书本，那些巧妙地机制原理生动地在脑中一一...

作为基础知识的梳理和学习之用，这本书无疑是很给力的。 从章节架构内含的相互联系可以看出作者和编辑很是努力了一把的。 任何具备一些常识的人想要进入分子生物学专业都可以从这本书的序章开始，一点一点，一步一步深入进去。 当合上书本，那些巧妙地机制原理生动地在脑中一一...

作为基础知识的梳理和学习之用，这本书无疑是很给力的。 从章节架构内含的相互联系可以看出作者和编辑很是努力了一把的。 任何具备一些常识的人想要进入分子生物学专业都可以从这本书的序章开始，一点一点，一步一步深入进去。 当合上书本，那些巧妙地机制原理生动地在脑中一一...

作为基础知识的梳理和学习之用，这本书无疑是很给力的。 从章节架构内含的相互联系可以看出作者和编辑很是努力了一把的。 任何具备一些常识的人想要进入分子生物学专业都可以从这本书的序章开始，一点一点，一步一步深入进去。 当合上书本，那些巧妙地机制原理生动地在脑中一一...

用户评价

这本书的排版和印刷质量简直是一场灾难，让我不禁怀疑这到底是不是一本正规出版物。纸张的手感粗糙得像砂纸，油墨的味道刺鼻得让人头疼，根本无法长时间阅读。更要命的是，图表的清晰度低到令人发指，那些关键的分子结构和实验流程图模糊不清，就像是隔着一层毛玻璃在看，完全无法帮助理解复杂的生物学概念。我翻阅了几个章节，发现排版混乱，段落之间缺乏必要的间距，使得大段文字堆砌在一起，视觉疲劳感极强。特别是在涉及电泳结果和测序图谱的那几部分，那些本应是黑白分明的线条和条带，在我手里这本“珍本”上，几乎融成了一团灰色的色块，我不得不借助放大镜，费了九牛二虎之力才能勉强分辨出个大概轮廓。老实说，如果不是课程要求必须使用这本教材，我绝对会毫不犹豫地把它扔进回收箱。这种低劣的制作工艺，不仅影响了阅读体验，更严重地削弱了教材应有的教学辅助功能，简直是对知识的亵渎，让学习过程充满了不必要的挫败感。我甚至怀疑，那些为了这本书的装帧和印刷投入的时间和金钱，如果能稍微投入到内容校对和图文优化上，这本书的价值都会提升好几个档次。

这本书的习题设计简直是故意的刁难，完全没有遵循任何合理的学习曲线或知识巩固的逻辑。有些问题过于宽泛和哲学化，要求你概括一个章节的全部内容，这种题型除了考验速记能力外，对真正理解机制毫无帮助；而另一些问题则过于偏执地纠结于某个实验的微小技术细节，比如某个特定缓冲液的pH值或特定酶的分子量，这些信息在正文中要么一带而过，要么根本没有明确提及，迫使读者不得不花费大量时间去查阅其他资料，这完全违背了教材自洽性的基本原则。更糟糕的是，这本书提供的答案解析极度简略，很多时候只是给出了最终结论，却完全没有展示推导过程或理论依据，这使得我们在自我检验时，即便做错了，也不知道错在哪里，更无法从中吸取教训。这种“问而不答”或“答非所问”的习题设计，极大地打击了读者的学习积极性，让人感觉学习过程更像是一场与书本的无望博弈，而非有益的探索之旅。

我发现这本书在内容更新速度上完全跟不上当代生物科学日新月异的步伐。尽管它力图覆盖基础理论，但在提及基因编辑技术、单细胞测序或者非编码RNA功能解析等热门且快速发展的领域时，其论述显得陈旧而保守，仿佛时间定格在了十年前。很多章节中引用的经典实验固然重要，但如果不能与最新的突破性发现和技术进步相结合，这些知识点就显得干瘪而缺乏生命力。我查阅了近期发表在权威期刊上的几篇综述，发现书中对某些争议性话题的处理方式也显得过于“教科书式”的定论化，缺乏对不同学派观点和仍在探索中的前沿问题的辩证性探讨。对于一个致力于了解“分子生物学”现状的读者来说，这本教材提供的知识更像是历史陈列品，而非通往未来的地图。如果我仅凭这本书来准备任何关于现代分子生物学的面试或研究讨论，我无疑会暴露自己的知识面严重滞后，这对于一本昂贵的教材来说，是不可原 বাসন的失职。

令人感到困惑的是，尽管这本书厚重如砖，内容包罗万象，但其对实验操作的实际指导性却异常薄弱。理论知识的阐述固然是基础，但对于生物学这种实践性极强的学科而言，如何将书本上的理论转化为实际操作是至关重要的一环。遗憾的是，书中对关键实验技术，比如PCR的优化条件、DNA克隆的载体选择依据，或者大规模蛋白质纯化的策略等，往往只是用几段文字草草带过，缺乏对“为什么”要选择这些步骤的深入解释，以及对常见实验失败原因的预警和排查指南。我尝试对照书中的描述去设计一个基础的分子克隆实验，结果发现很多实际操作中的“潜规则”和经验性的技巧完全没有被提及，这使得理论与实践之间存在着一道巨大的鸿沟。对于一个需要将所学知识应用于湿实验的初级研究人员来说，这本书的作用更像是一个高深的理论背景资料库，而非一本可以随时翻阅、指导操作的实用手册。它似乎假设读者已经掌握了所有的基本技术，这对于许多自学或基础课程学生来说，是极不负责任的疏忽。

这本书的叙事风格极其古板和说教，读起来就像是在啃一本厚重的、没有灵魂的法律条文集。作者似乎完全沉浸在自己构建的学术象牙塔中，使用了一堆晦涩难懂的、脱离实际语境的专业术语，却很少提供生动的例子或清晰的类比来辅助我们这些初学者建立直观的认知框架。很多地方，我需要反复阅读同一段落三四遍，才能勉强捕捉到作者想要表达的核心思想，但即便如此，理解也往往停留在字面意思的层面，缺乏深层的洞察力。更让人抓狂的是，它在介绍一个新概念时，常常跳过了必要的铺垫和背景知识的梳理，直接抛出一个复杂的模型或实验设计，仿佛我们这些读者都已经对前沿生物化学了如指掌。例如，在讨论基因表达调控机制时，书中对某些关键转录因子的作用机制描述得过于精炼，缺乏对动态平衡和负反馈循环的细致描绘，导致我对整个系统的运行逻辑始终感到一头雾水。这哪里是一本“教授知识”的书，分明更像是一份高门槛的专业人士内部参考手册，对那些渴望从零开始建立扎实基础的求知者来说，简直是冷漠且不友好的。

2007.08 | LIB | Watson主笔，令人热血沸腾的课本；很多令人惊奇的想法，除了些小bug，perfect

2007.08 | LIB | Watson主笔，令人热血沸腾的课本；很多令人惊奇的想法，除了些小bug，perfect

USWMB-SLUNK-KEVEL-FRIED-RIANT-PSHAM

2007.08 | LIB | Watson主笔，令人热血沸腾的课本；很多令人惊奇的想法，除了些小bug，perfect

2007.08 | LIB | Watson主笔，令人热血沸腾的课本；很多令人惊奇的想法，除了些小bug，perfect