Biological and medical research relies upon an integrated understanding of the molecules within cells and of the interactions between cells. This has imposed great demands on investigators. Being an expert in a relatively narrow area is no longer sufficient as many studies now require the use of a wide range of techniques to provide the necessary integration. A lack of familiarity with the experimental possibilities can make such diversification difficult to achieve. "Essential Cell Biology" is designed to help researchers overcome these problems. It has not been possible to include all of the techniques available in cell biology so the challenge was to identify those that might be most relevant to researchers who are new to this topic. The theory and basic principles of each technique are described, together with detailed protocols and advice for trouble shooting. Directions to more specialized techniques are also included.