Directed Evolution Library Creation

出版者:Humana Pr Inc

作者:Georgiou, George 编

页数:224

价格:$ 145.77

装帧:HRD

isbn号码:9781588292858

图书标签:专业书English

A comprehensive compendium of cutting-edge protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologus recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Copy for Both VolumesDirected Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.

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FA 的代表作

FA 的代表作

不仅内容丰富有趣，而且章节编辑得非常系统，从点突到shuffling到fusion chimera很漂亮。

不仅内容丰富有趣，而且章节编辑得非常系统，从点突到shuffling到fusion chimera很漂亮。

FA 的代表作