Following the considerable success of the first edition of "Plant Virology Protocols", this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section of "Plant Virology Protocols, Second Edition" presents the most novel methods and protocols to allow researchers more effective approaches to study plant viruses. The many subjects covered in this important new edition range from techniques for investigating viral nucleic acid sequence, protein analysis and investigation of protein function, techniques for microscopy/GFP visualization and analysis/protein tagging/generation of infectious clones and other such tools, as well as areas of genomics and interactions with host factors and plant-based studies. "Plant Virology Protocols, Second Edition" - from viral sequence to protein function is indispensable to laboratory investigators studying plant viruses.